Rapid Delivery of Gold Nanoparticles into Colon Cancer HT-29 Cells by Electroporation: In-vitro Study

Z Arab-Bafrani, D Shahbazi-Gahrouei, M Abbasian

Abstract


Introduction: Electroporation has become a routine technique for rapid drug delivery for the treatment of cancer. Because of its simplicity and wide range of application, it has been applied for the transfer of gold-nanoparticles and can facilitate entry into target cancer cells. The aim of this study is finding optimal conditions in order to obtain high GNPs- uptake and cell viability by means of electroporation.


Materials and Methods: Exponential electrical pulse with electric field intensity ranging from 0.2 -2 kV/cm, pulse length of 100 µs and the pulse number of 2 was used. Electroporated cell viability was investigated using MTS assay and GNPs-cellular uptake was assayed using graphite furnace atomic absorption spectrometry (GFAAS). Finally, electroporation results were compared with passive method.


Results and Conclusion: The maximum uptake occurred at 1.2 to 2 kV/cm and passive method happened. The cell viability of 1.2 kV/cm and passive method was about 90%, while the cell viability in 2 kV/cm drastically decreased to 50%. The findings showed that using two pulses of 1.2 kV/cm and 100 µs is a convenient way and surrogate of passive method for internalizing GNPs into cells.


Keywords


Gold nanoparticles, Electroporation, Drug delivery, HT-29

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DOI: https://doi.org/10.22086/jbpe.v0i0.579

eISSN: 2251-7200        JBPE NLM ID: 101589641

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                                                                        Chairman and Editor in Chief

                                                                              Dr. Alireza Mehdizadeh

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